Making transplants of Clytia colonies


It is easy to make a duplicate of a Clytia colony by making "cut", or "bouture" in french. We cut out a piece of colonies with one or more polyps from the strain you want to make duplicates and transplant it onto other substrates (beakers or glass plates).

When you make transplants?


  • Colonies are large enough and can afford to take several polyps.
  • To change substrate for the colonies, especially to switch to a larger plates etc.
  • There is no backup colonies for the strain.
  • A few weeks before sending off the strain.

Procedures

1. Confirm the polyps are well enough.

Efficiency will be lower if the polyps are starved. Feed enough to make them good in shape. Polyps should be big enough and catch artemia without helps.

2. Cut out polyps with a fine scissor.

Isolate individual polyps by cutting stem of polyps at proximity of stolon (bottom of the stem).
Some part of stolons in a colonies may not be attached to the substrate and growing vertically. In this case you can easily cut the stolons by scissors without damaging. Isolate a short piece of stolons with 2~3 well formed polyps.

3. Wash

Flush polyps with sea water together using a pipette (opening 3~4 mm) to get rid of algae as much as possible.

4. Transplant

Place isolated polyps on the target substrate in sea water and leave them quiet for 1~3 nights. Several pieces will be necessary for a large substrate (100 square-centimeter or more) or a few of them for a glass slide. Time required to attaching polyps depends on the strain. Well-growing Z4B polyps will attach in a night while Z4C2 polyps usually takes a few days. Confirm attachment by gently blowing sea water over the transplanted polyps.

5. Start normal culture

Slowly add sea water to the beaker (in case of beaker culture) or transfer the substrate to a culture tank. The attached polyps will extend stolons in good conditions of nutrition. Feeding therefore is very important for new transplants to let them attach stably. Feed artemia as soon as possible if the polyps are still capable to eat. Often the they degenerate polyps to give priority in extending stolons then reform a new polyps later on. Reformed polyps are usually smaller and the timing is unpredictable. Check such new polyp formation every day and feed very carefully. Bisected artemia (tail half) is good for small polyps. The new transplant can be treated as normal colony once they make 2nd or 3rd polyps in it.